Varicella zoster virus

Kit: EliGene® VZV RT Kit (Ref. 90040-RT)
Package size:
 50 reactions
Possibility of quantification:
 EliDNA VZV QRT standard
Analytical specificity: Varicella zoster virus
Analytical sensitivity: 1 – 10 copies of viral DNA in the amplified sample
Specimens: CSF, biopsy (skin, liver)
Compatible instruments:
 ABI 7000, ABI 7300, ABI 7500 (Applied Biosystems), LightCycler® 480 and LightCycler® Nano (ROCHE); fully compatible with RotorGene instruments; MyGo Mini, MyGo Pro (IT-IS Life Science)
CE certification: yes
Detected DNA region: genome
Detection technology: TaqMan probes
Clinical study description and results:
Within the frame of testing the functional characteristics of EliGene® VZV RT kit overall 481 clinical specimens were analyzed. From these 481 clinical specimens 51 were positive. EliGene® VZV RT kit analyzed all 51 samples as positive. Totally 430 clinical specimens were properly by EliGene® VZV RT kit analyzed as negative
Sensitivity: 100%
Specificity: 100%
Pathogen description:
VZV is a double stranded DNA virus of the Herpes group. VZV infection can have two different manifestations of the disease - chickenpox or shingles. The manifestations include rash, low grade fever and malaise. Varicella may rarely result in complications including VZV encephalitis or pneumonia. VZV is certainly transmissible through the airborne route and does not require close personal contact. The skin lesions are certainly full of infectious virus particles. After an incubation period of 14 days, the virus arrives at its main target organ, the skin. The virus can survive in the human body in the latent form for a long time.
References:
Bannister BA, Begg NT, Gillespie SH. 2000. Infectious Disease. Blackwell Science, 2th Ed.
Dwyer DE, Cunningham AL. 2002. Herpes simplex and varicella-zoster virus infections. Med J Aust. 177(5):267-73.
Hlinomazová Z, Loukotová V, Horáčková M, Serý O. 2010. The treatment of HSV1 ocular infections using quantitative real-time PCR results. Acta Ophthalmol. 10: 1755-3768.