Ureaplasma sp.

Kit: EliGene® Ureaplasma UNI Kit (ref: 90049-UNI)
Package size:
 50 reactions
Analytical specificity: Ureaplasma urealyticum, Ureaplasma parvum
Analytical sensitivity: 1 – 10 copies of bacterial DNA in the amplified sample
Specimens: urine, swabs (cervical, urethral)
Compatible instruments:
 ABI 7300, ABI 7500 (FAST) (Applied Biosystems); LightCycler® 480, LightCycler® Nano, LightCycler® 2.0 (Roche); RotorGene 6000 or RotorGene Q (Qiagen); MyGo Mini, MyGo Pro (IT-IS Life Science)
CE certification: yes
Detected DNA region: genome (ureA gene)
Detection technology: Molecular Beacons
Clinical study description and results:
Within the frame of testing the functional characteristics of Eligene Ureaplasma UNI Kit, totally 70 clinical specimens were analyzed. The EliGene Ureaplasma UNI Kit diagnosed correctly as Ureaplasma Urealyticum or Ureaplasma parvum positive 20 specimens. Totally 48 specimens were rightly determined by Eligene Ureaplasma UNI Kit as Ureaplasma Urealyticum or Ureaplasma parvum negative.
Sensitivity: 100%
Specificity: 100%
Pathogen description:
Ureaplasma urealyticum is bacterium belonging to the family Mycoplasmataceae. U. urealyticum consists of 14 serovars that can be divided into two biovars, biovars 1 and biovars 2, on the basis of genotypic characteristics. Evidence has been presented that the species currently known as U. urealyticum should be separated into two species, namely, U. parvum (previously, U. urealyticum biovar 1) and U. urealyticum (previously, U. urealyticum biovar 2). The majority of human Ureaplasma isolates belong to the new species U. parvum. Ureaplasma sp. is the main cause of non-gonococcal, non-chlamydial urethritis, acute prostatitis and acquired arthritis in men. In pregnant and non-pregnant women, Ureaplasma can cause chorioamnionitis and pre-term delivery, abortion, pre-term birth, vaginitis and cervicitis. Clinical studies have demonstrated that infants born to infected mothers become infected with these bacteria, and colonization of the respiratory tract of infants has been associated with pneumonia, respiratory distress and meningitis.
References:
Allam AB, Alvarez S, Brown MB, Reyes L. 2011. Ureaplasma parvum infection alters filamin A dynamics in host cells. BMC Infect Dis. 11:101.
Pinna GS, Skevaki CL, Kafetzis DA. 2006. The significance of Ureaplasma urealyticum as a pathogenic agent in the paediatric population. Curr Opin Infect Dis. 19(3):283-9.

Analysis of Clinical specimens by EliGene® Ureaplasm LC kit on LightCycler® 2.0 instrument including Positive control (PC)

FigureC