Multiplex Ureaplasma sp. & Mycoplasma hom/gen.

Kit: EliGene® Ureaplasma/Mycoplasma Kit (ref: 90073)
Package size:
 50 reactions
Analytical specificity: Ureaplasma urealyticum and parvum, Mycoplasma hominis,
Mycoplasma genitalium
Analytical sensitivity: 10 copies of viral DNA in the amplified sample
Specimens: urine, swabs (cervical, urethral, conjunctival, rectal), sperm
Compatible instruments:
 ABI 7500FAST instruments, LightCycler® 480, SmartCycler, Q-Gene instruments with five/six channels, MyGo® Pro cycler, BioRad CFX96® cycler
CE certification: coming soon (in 2017)
Detected DNA region: genome (porA gene - Ureaplasma; gap gene – M. hominis;
mgpB gene – M. genitalium)
Detection technology: TaqMan probes labelled with FAM, HEX, ROX and Cy5
fluorescence dyes
Pathogen description:
Ureaplasma urealyticum and Mycoplasma sp. are bacteria belonging to the family Mycoplasmataceae that are a group of microorganisms that can involve the respiratory and genital tracts in man to produce clinical disease.
U. urealyticum consists of 14 serovars that can be divided into two biovars, biovars 1 and biovars 2, on the basis of genotypic characteristics. Evidence has been presented that the species currently known as U. urealyticum should be separated into two species, namely, U. parvum (previously, U. urealyticum biovar 1) and U. urealyticum (previously, U. urealyticum biovar 2). The majority of human Ureaplasma isolates belong to the new species U. parvum. Ureaplasma sp. is the main cause of non-gonococcal, non-chlamydial urethritis, acute prostatitis and acquired arthritis in men. In pregnant and non-pregnant women, Ureaplasma can cause chorioamnionitis and pre-term delivery, abortion, pre-term birth, vaginitis and cervicitis.
Mycoplasma hominis is associated with pelvic inflammatory disease, bacterial vaginitis, post-partum fever, sepsis and infections of the central nervous system often leading to serious conditions.
In case of Mycoplasma genitalium there is strong evidence of having a causative role in non-chlamydia, non-gonococcal urethritis (NCNGU) in men and cervicitis in women. M. genitalium infection has also been associated with pelvic inflammatory disease but the exact role has not yet been determined.
References:
Allam AB, Alvarez S, Brown MB, Reyes L. 2011. Ureaplasma parvum infection alters filamin A dynamic in host cells. BMC Infect Dis. 11:101.

Baczynska A, Svenstrup HF, Fedder J, Birkelund S, Christiansen G. 2004. Development of real-time PCR for detection of Mycoplasma hominis. BMC Microbiology, 4:35.

Edberg A, Jurstrand M, Johansson E, Wikander E,Höög A, Ahlqvist T, Falk L, Skov J, Fredlund H. 2008. A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women. Journal of Medical Microbiology, 57, 304–309.

Pinna GS, Skevaki CL, Kafetzis DA. 2006. The significance of Ureaplasma urealyticum as a pathogenic agent in the paediatric population. Curr Opin Infect Dis. 19(3):283-9.

Simpson T, Oh MK. 2004. Urethritis and cervicitis in adolescents. Adolesc Med Clin. 15(2):253-71.