EliGene TBEV (Tick-borne encephalitis virus)

Kit: EliGene® TBEV LC Kit (ref: 90070-LC)
Package size:
 50 reactions
Analytical specificity: Genome RNA of tick-borne encephalitis (TBE) virus
Analytical sensitivity: 50 copies of viral RNA in the amplified sample
Specimens: CSF, serum, plasma, ticks
Compatible instruments:
 ABI 7300, ABI 7500, ABI 7500FAST (Applied Biosystems), LightCycler® 480 and LightCycler® Nano (ROCHE); fully compatible with RotorGene instruments; MyGo Mini, MyGo Pro (IT-IS Life Science); SmartCycler; Bio-Rad CFX96®
CE certification: for veterinary use or research only
Detected DNA region: Genome (NS5 gene)
Detection technology: TaqMan probes labelled with FAM and HEX fluorescence dyes
Pathogen description:
Tick-borne encephalitis virus (TBEV) is the causative agent of tick-borne encephalitis (TBE), a human viral infectious disease involving the central nervous system. TBEV belongs to the genus Flavivirus within the family Flaviviridae. Up today three main subtypes of the virus were described: European, Siberian and far-eastern. Ticks, specifically hard ticks of the family Ixodidae, act as both the vector and reservoir for TBEV. TBE cases occur in humans most frequently in countryside and during the highest period of tick activity (between April and November). Infection also may follow consumption of raw milk from infected goats, sheep, or cows. The incubation period is generally 7–14 days when symptoms during the initial short febrile period can include fatigue, headache and pain in the neck, shoulders and lower back, accompanied by high fever and vomiting. This initial period is often followed by an asymptomatic period and during the second period disease characterized by acute CNS symptoms with a high fever.
References:
Mansfield, K.L., Johnson, N., Phipps, L.P., Stephenson, J.R., Fooks, A.R., and Solomon, T. (2009). Tick-borne encephalitis virus – a review of an emerging zoonosis. J Gen Virol 90, 1781–1794

Lindquist, L., Vapalahti, O. (2008). Tick-borne encephalitis. Lancet 371(9627), 1861-71

Suess, J. (2008) Tick-borne encephalitis in Europe and beyond -- the epidemiological situation as of 2007. Euro Surveillance 13(26)