Norovirus

Kits: EliGene® Norovirus LC Kit (ref: 90054-RT)
Package size:
 50 reactions
Analytical specificity: Norovirus genogroup 1 and 2
Analytical sensitivity: 1 – 10 viruses in the amplified sample
Specimens: serum, plasma, feces, water, CSF
Compatible instruments:
 ABI 7500 (Applied Biosystems), LightCycler® 480 and LightCycler® Nano, LightCycler® 2.0 (Roche), RotorGene 6000 or RotorGene Q (Qiagen); MyGo Mini, MyGo Pro (IT-IS Life Science)
CE certification: yes
Detected DNA region: genome
Detection technology: Molecular Beacons probes
Detection technology description:
The EliGene® Norovirus LC kit is intended for detection and quantification of Norovirus RNA. Kit contains reversion transcription in one mix with RealTime detection.
Clinical study description and results:
Within the frame of testing functional characteristics of the EliGene® Norovirus LC kit overall 100 clinical specimens were analyzed. From these specimens 100 blind specimens were verified by reference method (La Rosa et al. 2010). From these 100 samples 56 samples were Norovirus RNA positive. The EliGene® Norovirus LC kit diagnosed as Norovirus RNA positive all 56 specimens. There were no discrepant results. Totally 44 specimens were right determined by the EliGene® Norovirus LC kit as Norovirus RNA negative.
Sensitivity: 100 %
Specificity: 100 %
Pathogen description:
Noroviruses are a genetically diverse group of single-stranded RNA, non enveloped viruses in the Caliciviridae family. The viruses are transmitted by fecally contaminated food or water, by person-to-person contact, and via aerosolization of the virus and subsequent contamination of surfaces. Noroviruses are the most common cause of viral gastroenteritis in humans. Norovirus affects people of all ages. Outbreaks of norovirus infection often occur in closed or semiclosed communities, such as long-term care facilities, overnight camps, hospitals, prisons, dormitories, and cruise ships, where the infection spreads very rapidly either by person-to-person transmission or through contaminated food. Norovirus is rapidly inactivated by either sufficient heating or by chlorine-based disinfectants, but the virus is less susceptible to alcohols and detergents, as it does not have a lipid envelope.
References:
BannisterBA, BeggNT, Gillespie SH. 2000. Infectious Disease. Blackwell Science, 2th Ed.

Oberste MS, Maher K, Kilpatrick DR, Pallansch MA. 1999. Molecular evolution of the human Noroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification". J. Virol. 73 (3):1941–8