Neisseria gonorrhoeae

Kit: EliGene® Neisseria UNI Kit (ref: 90047-UNI)
Package size:
 50 reactions
Analytical specificity: Neisseria gonorrhoeae
Analytical sensitivity: 1 – 10 copies of bacterial DNA in the amplified sample
Specimens: urine, swabs (cervical, urethral)
Compatible instruments:
 ABI 7300, ABI 7500 (FAST) (Applied Biosystems); LightCycler® 480, LightCycler® Nano, LightCycler® 2.0 (Roche); RotorGene 6000 or RotorGene Q (Qiagen); MyGo Mini, MyGo Pro (IT-IS Life Science)
CE certification: yes
Detected DNA region: genome (porA gene)
Detection technology: Molecular Beacons
Clinical study description and results:
88 Clinical samples were analysed and the results verified using the Cobas Amplicor CT/NG test. The EliGene® Neisseria kit correctly identified 35 samples as positive and 53 samples as negative for N. gonorrhoeae.
Sensitivity: 100%
Specificity: 100%
Pathogen description:
Neisseria gonorrhoeae (gonococci) are gram-negative, non-motile and non-sporulated diplococci. Of the eleven species of Neisseria that colonize humans, only two are pathogens, N. gonorrhoeae and N. meningitis. N. gonorrhoeae is the causative agent of sexually transmitted disease gonorrhoea that is the third most common sexual disease. Worldwide, about 62,2 millions cases of gonorrhoea are registered. The simultaneous infection by Chlamydia trachomatis is common.
References:
Whiley DM, Garland SM, Harnett G, Lum G, Smith DW, Tabrizi SN, Sloots TP, Tapsall JW. 2008. Exploring 'best practice' for nucleic acid detection of Neisseria gonorrhoeae. Sex Health. 5(1):17-23.
Whiley DM, Buda PP, Freeman K, Pattle NI, Bates J, Sloots TP. 2005. A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens. Diagn Microbiol Infect Dis. 52(1):1-5.