EliGene Herpes simplex virus 1/2

Kit: EliGene® HSV1/HSV2 UNI Kit (Ref: 90062-UNI)
Package size:
 50 reactions
Analytical specificity: Herpes simplex virus 1 and 2
Analytical sensitivity: 1 – 10 copies of viral DNA in the amplified sample
Specimens: CSF, biopsy, swabs (skin, mucosa), blood
Compatible instruments:
 ABI 7000, ABI 7300, ABI 7500 (Applied Biosystems), LightCycler® 480 and LightCycler® Nano (ROCHE); fully compatible with RotorGene instruments; MyGo Mini, MyGo Pro (IT-IS Life Science)
CE certification: yes
Detected DNA region: genome (US4 gene)
Detection technology: Molecular beacons
Clinical study description and results:
Within the frame of testing the functional characteristics of EliGene® HSV1/HSV2 UNI kit overall 847 clinical specimens were analyzed. From these specimens EliGene® HSV1/HSV2 UNI kit analyzed 197 samples as HSV1 positive and 24 samples as HSV2 positive. In four positive specimens for HSV1 virus an inhibition of the test occurred. Totally 622 clinical specimens were properly by EliGene®HSV1/HSV2 UNI kit analyzed as HSV1/HSV2 negative.
Sensitivity: 99,5 %
Specificity: 100 %
Pathogen description:
Herpesvirus is enveloped DNA virus. To date, at least eight different herpesvirus species are known to infect man.
A primary HSV1 infection typically occurs during the childhood. The primary infection goes often through symptomless. The HSV1 infection can manifest as an undifferentiational catarrh of respiratory system of adults or older children. The infection of the conjunctiva and cornea manifests as keratoconjunctivitis.
HSV2 causes genital herpes, characterized by sores in the area of the genitalia. Genital herpes is a sexually transmitted disease. The main symptoms are herpes ulcers in glans penis in men. Lesions in woman are localized in cervix, vagina or perianal area. The activation of the latent infection can produce asymptomatic releasing of virus in cervical secret. HSV2 can cause infection of the brain (encephalitis) in the case of severely defective or compromised immune system.
References:
Bannister BA, Begg NT, Gillespie SH. 2000. Infectious Disease. Blackwell Science, 2th Ed.
Hlinomazová Z, Loukotová V, Horáčková M, Serý O. 2010. The treatment of HSV1 ocular infections using quantitative real-time PCR results. Acta Ophthalmol. 10: 1755-3768.