Enterovirus

Kit: EliGene® Enterovirus LC Kit (ref: 90053-LC) 
Package size:
 50 reactions
Analytical specificity: Enteroviruses (Coxsackie A, Coxsackie B and Echovirus)
Analytical sensitivity: 1 – 10 copies of viral RNA in the amplified sample
Specimens: CSF, feces, respiratory samples, pericardial fluid, blood
Compatible instruments:
 ABI 7500 (Applied Biosystems), LightCycler® 480 and LightCycler® Nano, LightCycler® 2.0 (Roche), RotorGene 6000 (Qiagen); MyGo Mini, MyGo Pro (IT-IS Life Science)
CE certification: yes
Detected DNA region: genome
Detection technology: Molecular Beacon
Clinical study description and results:
Within the frame of testing functional characteristics of the EliGene® Enterovirus LC kit overall 100 clinical specimens were analyzed. From these specimens 100 blind specimens were verified by reference method (La Rosa et al. 2010). From these 100 samples 56 samples were Enterovirus RNA positive. The EliGene® Enterovirus LC kit diagnosed as Enterovirus RNA positive all 56 specimens. There were no discrepant results. Totally 44 specimens were right determined by the EliGene® Enterovirus LC kit as Enterovirus RNA negative.
Sensitivity: 100 %
Specificity: 100 %
Pathogen description:
Human Enteroviruses are a genus of (+)ssRNA viruses associated with several human and mammalian diseases. Until now, 66 serotypes are known of which there are 62 non-polio enteroviruses that can cause disease in humans: 23 Coxsackie A viruses, 6 Coxsackie B viruses, 28 echoviruses, and 5 other enteroviruses. Enteroviruses are ubiquitous pathogens with a high incidence worldwide (ca. 500 million infections/year) and are often found in the respiratory secretions (e.g., saliva, sputum, or nasal mucus) and stool of an infected person.  Enteroviruses may cause life-threatening infections, especially among children when diseases such as myocarditis, paralysis, multiple organ failure, meningitis and encephalitis may be associated with infections.
References:
Bannister BA, Begg NT, Gillespie SH. 2000. Infectious Disease. Blackwell Science, 2th Ed.
Oberste MS, Maher K, Kilpatrick DR, Pallansch MA. 1999. Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification". J. Virol. 73 (3): 1941–8.