Adenovirus

Kit: EliGene® Adenovirus RT Kit (ref: 90036-RT) 
Package size:
 50 reactions
Possibility of quantification:
 EliDNA ADV QRT standard
Analytical specificity: all 51 types of Adenoviruses
Analytical sensitivity: 1 – 10 copies of viral DNA in the amplified sample
Specimens: urine, CSF, feces, swabs
Compatible instruments:
 ABI 7000, ABI 7300, ABI 7500 (Applied Biosystems), LightCycler® 480 and LightCycler® Nano (ROCHE); fully compatible with RotorGene instruments; MyGo Mini, MyGo Pro (IT-IS Life Science)
CE certification: yes
Detected DNA region: genome (37 hexon gene)
Detection technology: TaqMan probes
Clinical study description and results:
Within the frame of testing the functional characteristics of EliGene® Adenovirus RT kit overall 350 clinical specimens were analyzed. From these 350 clinical specimens were 20 positive. EliGene® Adenovirus RT kit analyzed 20 samples as positive. Totally 330 clinical specimens were properly by EliGene® Adenovirus RT kit analyzed as negative.
Sensitivity: 100%
Specificity: 100%
Pathogen description:
Adenoviruses represent the largest nonenveloped viruses. They are able to be transported through the endosome. They are about 70 to 90 nm in size, nonenveloped icosahedral viruses that are made from up to 252 capsomeres containing double-stranded DNA. Infection is usually transmitted by droplets of respiratory or ocular secretions, by alimentary transfer or sexual intercourse and by the contact with contaminated things or water (virus is able to live out of body at temperature 20°C and lower for a few weeks). The places of the first reproduction are the most often cells of epithelial conjunctiva, nasopharinx and intestine. Adenovirus infection is clinically manifested by the fever, upper respiratory tract infections, tonsillitis, laryngitis, bronchitis and pneumonia.
References:
Bannister BA, Begg NT, Gillespie SH. 2000. Infectious Disease. Blackwell Science, 2th Ed.
Jothikumar N, Cromeans TL, Hill VR, Lu X, Sobsey MD, Erdman DD. 2005. Quantitative real-time PCR assays for detection of human adenoviruses and identification of serotypes 40 and 41. Appl Environ Microbiol. 71(6):3131-6.
Hlinomazová Z, Loukotová V, Horáčková M, Serý O. 2010. The treatment of HSV1 ocular infections using quantitative real-time PCR results. Acta Ophthalmol. 10: 1755-3768.